Dotted lines indicate the total number of ASVs/OTUs expected, accounting for 16S copy variation within genomes. ![]() “Unmatched” sequences did not match an expected sequence or the SILVA/ITS databases at 97% identity or greater. Figure S4: Total number of ASVs/OTUs identified by each sequence processing method for four different mock communities after removal of low abundance ASVs/OTUs reveals large shift in number of OTUs Amplicon sequence variants/operational taxonomic units (ASVs/OTUs) were run through an abundance filtering at 0.1% (HMP, Fungal, Zymomock) or 0.0004% (Extreme) remaining ASVs/OTUs were compared to a database of full-length amplicon sequences for just the microbes supposedly in the community (“Expected”) and against the full SILVA or ITS databases (“Database”) using BLASTN at 97% and 100% identity cut-offs.
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